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cell signalling technology 8707  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc cell signalling technology 8707
    Cell Signalling Technology 8707, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+map2/MAP2+XP+Rabbit+mAb/pm41915835-200-53-53
    Average 96 stars, based on 248 article reviews
    cell signalling technology 8707 - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Tau oligomers modulate synapse fate by eliciting progressive bipartite synapse dysregulation and synapse loss.
    Article Snippet: .. 245 Antibodies 246 Primary antibodies used for western blot and immunocytochemistry were: Tau5 247 (AB_80579, Abcam), Streptavin HRP (S911, Invitrogen), GAPDH (MAB374, Sigma), 248 Rabbit FLAG (F7425, Sigma), Mouse FLAG (F3165, Sigma), PSD-95 (MA1046, 249 ThermoFisher), GluA2/3 (AB1506, MilliporeSigma), Cy3 Streptavidin (AB_2337244, 250 Jackson Immunoresearch), Mouse vGluT1 (MAB5502, MilliporeSigma), Guinea Pig 251 vGluT1 (AB5905, MilliporeSigma), GluA1 (ABN241, MilliporeSigma), GluN1 (114 011, ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS 12 252 SynapticSystems), Synapsin (5297S, Cell Signaling), Rabbit MAP2 (4542S, Cell 253 Signaling), Chicken MAP2 (NB300 213, Novus Biologicals), NeuN (AB177487, Abcam), 254 GFAP (PA110004, Invitrogen), Rabbit GSK3b (ab32391, Abcam). .. 255 Secondary antibodies used were: Anti-mouse Alexa Fluor 488 (115-545-166, 256 Jackson Immunoresearch), Anti-guinea pig Alexa Fluor 546 (A-11074, Life 257 Technologies), Anti-mouse Alexa Fluor 546 (A-11030, Invitrogen), Anti-rabbit Alexa 258 Fluor 647 (111-605-144, Jackson Immunoresearch), Anti-chicken Alexa Fluor 647 259 (A32933, Invitrogen).

    Article Title: Tau oligomers modulate synapse fate by eliciting progressive bipartite synapse dysregulation and synapse loss
    Article Snippet: .. Primary antibodies used for western blot and immunocytochemistry were: Tau5 (AB_80579, Abcam), Streptavin HRP (S911, Invitrogen), GAPDH (MAB374, Sigma), Rabbit flag (F7425, Sigma), Mouse flag (F3165, Sigma), PSD-95 (MA1046, ThermoFisher), GluA2/3 (AB1506, MilliporeSigma), Cy3 Streptavidin (AB_2337244, Jackson Immunoresearch), Mouse vGluT1 (MAB5502, MilliporeSigma), Guinea Pig vGluT1 (AB5905, MilliporeSigma), GluA1 (ABN241, MilliporeSigma), GluN1 (114 011, SynapticSystems), Synapsin (5297 S, Cell Signaling), Rabbit MAP2 (4542 S, Cell Signaling), Chicken MAP2 (NB300 213, Novus Biologicals), NeuN ( AB177487 , Abcam), GFAP ( PA110004 , Invitrogen), Rabbit GSK3β (ab32391, Abcam), and MYO5A ( AB244249 , Abcam). .. Secondary antibodies used were: Anti-mouse Alexa Fluor 488 (115-545-166, Jackson Immunoresearch), Anti-guinea pig Alexa Fluor 546 (A-11074, Life Technologies), Anti-mouse Alexa Fluor 546 (A-11030, Invitrogen), Anti-rabbit Alexa Fluor 647 (111-605-144, Jackson Immunoresearch), Anti-chicken Alexa Fluor 647 (A32933, Invitrogen).

    Immunocytochemistry:

    Article Title: Tau oligomers modulate synapse fate by eliciting progressive bipartite synapse dysregulation and synapse loss.
    Article Snippet: .. 245 Antibodies 246 Primary antibodies used for western blot and immunocytochemistry were: Tau5 247 (AB_80579, Abcam), Streptavin HRP (S911, Invitrogen), GAPDH (MAB374, Sigma), 248 Rabbit FLAG (F7425, Sigma), Mouse FLAG (F3165, Sigma), PSD-95 (MA1046, 249 ThermoFisher), GluA2/3 (AB1506, MilliporeSigma), Cy3 Streptavidin (AB_2337244, 250 Jackson Immunoresearch), Mouse vGluT1 (MAB5502, MilliporeSigma), Guinea Pig 251 vGluT1 (AB5905, MilliporeSigma), GluA1 (ABN241, MilliporeSigma), GluN1 (114 011, ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS 12 252 SynapticSystems), Synapsin (5297S, Cell Signaling), Rabbit MAP2 (4542S, Cell 253 Signaling), Chicken MAP2 (NB300 213, Novus Biologicals), NeuN (AB177487, Abcam), 254 GFAP (PA110004, Invitrogen), Rabbit GSK3b (ab32391, Abcam). .. 255 Secondary antibodies used were: Anti-mouse Alexa Fluor 488 (115-545-166, 256 Jackson Immunoresearch), Anti-guinea pig Alexa Fluor 546 (A-11074, Life 257 Technologies), Anti-mouse Alexa Fluor 546 (A-11030, Invitrogen), Anti-rabbit Alexa 258 Fluor 647 (111-605-144, Jackson Immunoresearch), Anti-chicken Alexa Fluor 647 259 (A32933, Invitrogen).

    Article Title: Tau oligomers modulate synapse fate by eliciting progressive bipartite synapse dysregulation and synapse loss
    Article Snippet: .. Primary antibodies used for western blot and immunocytochemistry were: Tau5 (AB_80579, Abcam), Streptavin HRP (S911, Invitrogen), GAPDH (MAB374, Sigma), Rabbit flag (F7425, Sigma), Mouse flag (F3165, Sigma), PSD-95 (MA1046, ThermoFisher), GluA2/3 (AB1506, MilliporeSigma), Cy3 Streptavidin (AB_2337244, Jackson Immunoresearch), Mouse vGluT1 (MAB5502, MilliporeSigma), Guinea Pig vGluT1 (AB5905, MilliporeSigma), GluA1 (ABN241, MilliporeSigma), GluN1 (114 011, SynapticSystems), Synapsin (5297 S, Cell Signaling), Rabbit MAP2 (4542 S, Cell Signaling), Chicken MAP2 (NB300 213, Novus Biologicals), NeuN ( AB177487 , Abcam), GFAP ( PA110004 , Invitrogen), Rabbit GSK3β (ab32391, Abcam), and MYO5A ( AB244249 , Abcam). .. Secondary antibodies used were: Anti-mouse Alexa Fluor 488 (115-545-166, Jackson Immunoresearch), Anti-guinea pig Alexa Fluor 546 (A-11074, Life Technologies), Anti-mouse Alexa Fluor 546 (A-11030, Invitrogen), Anti-rabbit Alexa Fluor 647 (111-605-144, Jackson Immunoresearch), Anti-chicken Alexa Fluor 647 (A32933, Invitrogen).

    Staining:

    Article Title: Aberrant neuronal firing: a paracrine route to glioblastoma expansion.
    Article Snippet: .. In brief, the cells were fixed with 4% Para overnight, followed by permeabilization with 0.1% Triton X-100 for 5 min. Neuronal purity was assessed by staining by primary antibodies of rabbit-MAP2 (1:200) (4542 S, Cell signaling technology®, Danvers, MA, USA), and mouse-GFAP (1:200) (G3893, Sigma Aldrich), and secondary antibody of anti-mouse 488 Alexa Fluor (1:400) (Thermo Fisher Science) and anti-rabbit 546 Alexa Fluor (1:400) (Invitrogen), to distinguish neurons from glial cells. .. For glutamate immunocytochemistry based on the description of the prior studies [92, 93], STAINperfrect Immunostaining Kit A (SP-A-1000, ImmuSmol, Bordeaux, France) was used according to the protocol of the manufacturer to detect L-glutamine.

    Article Title: Aberrant neuronal firing: a paracrine route to glioblastoma expansion
    Article Snippet: .. In brief, the cells were fixed with 4% Para overnight, followed by permeabilization with 0.1% Triton X-100 for 5 min. Neuronal purity was assessed by staining by primary antibodies of rabbit-MAP2 (1:200) (4542 S, Cell signaling technology ® , Danvers, MA, USA), and mouse-GFAP (1:200) (G3893, Sigma Aldrich), and secondary antibody of anti-mouse 488 Alexa Fluor (1:400) (Thermo Fisher Science) and anti-rabbit 546 Alexa Fluor (1:400) (Invitrogen), to distinguish neurons from glial cells. .. For glutamate immunocytochemistry based on the description of the prior studies [ , ], STAINperfrect Immunostaining Kit A (SP-A-1000, ImmuSmol, Bordeaux, France) was used according to the protocol of the manufacturer to detect L-glutamine.

    Blocking Assay:

    Article Title: Membrane interfacial potential governs surface condensation and fibrillation of α-Synuclein in neurons.
    Article Snippet: .. The primary antibodies, rabbit MAP2 (1:150, Cell Signaling Technology, #8707S), Guinea pig Synapsin1 (1:300, Synaptic Systems, #106104), chicken βIII-tubulin (1:200, Abcam, #AB41489), mouse α-synuclein (1:100, Invitrogen, #328100) and Rabbit anti-Myc of dilution (1:1000, Invitrogen, #PA1-981) were diluted in in the blocking solution (1% BSA) and incubated overnight at 4°C. ..

    Incubation:

    Article Title: Membrane interfacial potential governs surface condensation and fibrillation of α-Synuclein in neurons.
    Article Snippet: .. The primary antibodies, rabbit MAP2 (1:150, Cell Signaling Technology, #8707S), Guinea pig Synapsin1 (1:300, Synaptic Systems, #106104), chicken βIII-tubulin (1:200, Abcam, #AB41489), mouse α-synuclein (1:100, Invitrogen, #328100) and Rabbit anti-Myc of dilution (1:1000, Invitrogen, #PA1-981) were diluted in in the blocking solution (1% BSA) and incubated overnight at 4°C. ..



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    Waveform rescue and impairment of AIS in C9-MSNs (A) Example AP waveforms for Con-1 (blue) and C9-3 MSNs (black) before and after the addition of BK channel activator NS11021 (10 μM, lighter color shade). Scale bars, 20 mV, 10 ms. (B and C) Mean ± SEM AP amplitude, AHP, half-width, threshold, and rheobase of C9-3 MSNs in the presence and absence of NS11021, along with Con-1 MSNs, were examined in parallel. Statistics, ∗ p < 0.05 and ∗∗∗ p < 0.001, from paired t test or t test . Data: con-1: n = 8, N = 3. C9-3: n = 10, N = 4. (D) NS11021 treatment had no effect on AP output in C9-3 MSNs evoked from current stimulation. Con-1 patched in parallel, shown for reference. Data: Con-1: n = 8, N = 3. C9-3: n = 10, N = 4. Statistics, two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test. (E) Images of Con-1 and C9-3 stained with AIS marker ankyrin-G, neuronal marker <t>MAP2,</t> and DAPI. Scale bars, 50 μm. (F) Mean ± SEM AIS length and distance of start of AIS from the cell soma. Data: Con-1: n = 16, N = 4. C9-3: n = 36, N = 4. ∗∗∗ p < 0.001, t test.
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    OMT reversed the neurons damage and mitophagy reduction in the hippocampal of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of <t>MAP2,</t> PSD95 and SYN, n = 5; (C) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (D) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (E,F) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin.
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    Image Search Results


    Waveform rescue and impairment of AIS in C9-MSNs (A) Example AP waveforms for Con-1 (blue) and C9-3 MSNs (black) before and after the addition of BK channel activator NS11021 (10 μM, lighter color shade). Scale bars, 20 mV, 10 ms. (B and C) Mean ± SEM AP amplitude, AHP, half-width, threshold, and rheobase of C9-3 MSNs in the presence and absence of NS11021, along with Con-1 MSNs, were examined in parallel. Statistics, ∗ p < 0.05 and ∗∗∗ p < 0.001, from paired t test or t test . Data: con-1: n = 8, N = 3. C9-3: n = 10, N = 4. (D) NS11021 treatment had no effect on AP output in C9-3 MSNs evoked from current stimulation. Con-1 patched in parallel, shown for reference. Data: Con-1: n = 8, N = 3. C9-3: n = 10, N = 4. Statistics, two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test. (E) Images of Con-1 and C9-3 stained with AIS marker ankyrin-G, neuronal marker MAP2, and DAPI. Scale bars, 50 μm. (F) Mean ± SEM AIS length and distance of start of AIS from the cell soma. Data: Con-1: n = 16, N = 4. C9-3: n = 36, N = 4. ∗∗∗ p < 0.001, t test.

    Journal: Cell Reports

    Article Title: Striatal neuron dysfunction in C9ORF72-FTD/ALS is driven by AIS and potassium channel dysregulation

    doi: 10.1016/j.celrep.2026.117672

    Figure Lengend Snippet: Waveform rescue and impairment of AIS in C9-MSNs (A) Example AP waveforms for Con-1 (blue) and C9-3 MSNs (black) before and after the addition of BK channel activator NS11021 (10 μM, lighter color shade). Scale bars, 20 mV, 10 ms. (B and C) Mean ± SEM AP amplitude, AHP, half-width, threshold, and rheobase of C9-3 MSNs in the presence and absence of NS11021, along with Con-1 MSNs, were examined in parallel. Statistics, ∗ p < 0.05 and ∗∗∗ p < 0.001, from paired t test or t test . Data: con-1: n = 8, N = 3. C9-3: n = 10, N = 4. (D) NS11021 treatment had no effect on AP output in C9-3 MSNs evoked from current stimulation. Con-1 patched in parallel, shown for reference. Data: Con-1: n = 8, N = 3. C9-3: n = 10, N = 4. Statistics, two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test. (E) Images of Con-1 and C9-3 stained with AIS marker ankyrin-G, neuronal marker MAP2, and DAPI. Scale bars, 50 μm. (F) Mean ± SEM AIS length and distance of start of AIS from the cell soma. Data: Con-1: n = 16, N = 4. C9-3: n = 36, N = 4. ∗∗∗ p < 0.001, t test.

    Article Snippet: MAP2 anti-Rabbit (1:1000) , Synaptic Systems , Cat# 188 003; RRID AB_2281442.

    Techniques: Staining, Marker

    OMT reversed the neurons damage and mitophagy reduction in the hippocampal of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of MAP2, PSD95 and SYN, n = 5; (C) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (D) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (E,F) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin.

    Journal: Frontiers in Pharmacology

    Article Title: Oxymatrine alleviates cognitive dysfunction in mice caused by chronic cerebral hypoperfusion by promoting SIRT1/ PINK1-mediated mitophagy

    doi: 10.3389/fphar.2026.1736022

    Figure Lengend Snippet: OMT reversed the neurons damage and mitophagy reduction in the hippocampal of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of MAP2, PSD95 and SYN, n = 5; (C) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (D) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (E,F) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin.

    Article Snippet: The antibodies of microtubule-associated protein 2 (MAP2, A2572), postsynaptic density protein-95 (PSD95, A6194), synaptophysin (SYN, A6344) and sirtuin 1 (SIRT1, A11267) were sourced from Abclonal Technology Co., Ltd., Wuhan, China.

    Techniques: Immunofluorescence, Expressing, Electron Microscopy, Control

    OMT restored the mitophagy reduction and the SIRT1 inhibition in the hippocampus of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of PINK1and PARKIN, n = 5; (C) The mRNA expression of PINK1and PARKIN, n = 6; (D) Binding energy of OMT with downstream targets and binding sites of OMT to SIRT1; (E,F) Representative immunofluorescence images and quantitative analysis (n = 5), mRNA expression (n = 6) of SIRT1; (G,H) The protein expression and quantitative analysis of PINK1, PARKIN and SIRT1, n = 3. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Journal: Frontiers in Pharmacology

    Article Title: Oxymatrine alleviates cognitive dysfunction in mice caused by chronic cerebral hypoperfusion by promoting SIRT1/ PINK1-mediated mitophagy

    doi: 10.3389/fphar.2026.1736022

    Figure Lengend Snippet: OMT restored the mitophagy reduction and the SIRT1 inhibition in the hippocampus of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of PINK1and PARKIN, n = 5; (C) The mRNA expression of PINK1and PARKIN, n = 6; (D) Binding energy of OMT with downstream targets and binding sites of OMT to SIRT1; (E,F) Representative immunofluorescence images and quantitative analysis (n = 5), mRNA expression (n = 6) of SIRT1; (G,H) The protein expression and quantitative analysis of PINK1, PARKIN and SIRT1, n = 3. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Article Snippet: The antibodies of microtubule-associated protein 2 (MAP2, A2572), postsynaptic density protein-95 (PSD95, A6194), synaptophysin (SYN, A6344) and sirtuin 1 (SIRT1, A11267) were sourced from Abclonal Technology Co., Ltd., Wuhan, China.

    Techniques: Inhibition, Immunofluorescence, Expressing, Binding Assay, Control

    OMT reversed the OGD/R induced HT22 cell dysfunction, mitophagy inhibition and decreased SIRT1 expression. (A) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (B) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (C) The mRNA expression of PINK1, PARKIN and SIRT1, n = 6; (D) The protein expression and quantitative analysis of PINK1, PARKIN and SIRT1, n = 3; (E) Detection of JC-1 aggregates (red) and monomers (green) by confocal fluorescence microscopy, 1000×, n = 3; (F) Colocalization of lysosome (green) and mitochondria (red) by confocal fluorescence microscopy, 400×, 1000×, n = 3; (G) Representative ROS images, 100×, n = 5. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Journal: Frontiers in Pharmacology

    Article Title: Oxymatrine alleviates cognitive dysfunction in mice caused by chronic cerebral hypoperfusion by promoting SIRT1/ PINK1-mediated mitophagy

    doi: 10.3389/fphar.2026.1736022

    Figure Lengend Snippet: OMT reversed the OGD/R induced HT22 cell dysfunction, mitophagy inhibition and decreased SIRT1 expression. (A) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (B) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (C) The mRNA expression of PINK1, PARKIN and SIRT1, n = 6; (D) The protein expression and quantitative analysis of PINK1, PARKIN and SIRT1, n = 3; (E) Detection of JC-1 aggregates (red) and monomers (green) by confocal fluorescence microscopy, 1000×, n = 3; (F) Colocalization of lysosome (green) and mitochondria (red) by confocal fluorescence microscopy, 400×, 1000×, n = 3; (G) Representative ROS images, 100×, n = 5. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Article Snippet: The antibodies of microtubule-associated protein 2 (MAP2, A2572), postsynaptic density protein-95 (PSD95, A6194), synaptophysin (SYN, A6344) and sirtuin 1 (SIRT1, A11267) were sourced from Abclonal Technology Co., Ltd., Wuhan, China.

    Techniques: Inhibition, Expressing, Fluorescence, Microscopy, Control

    SIRT1 and mitophagy mediate the protective effect of OMT on OGD/R HT22 cells. (A) The mRNA expression of MAP2, PSD95, SYN PINK1 and PARKIN, n = 6; (B,C) The protein expression and quantitative analysis of MAP2, PSD95, SYN PINK1 and PARKIN, n = 3; (D) Detection of JC-1 aggregates (red) and monomers (green) by confocal fluorescence microscopy, 1000×, n = 3; (E) Colocalization of lysosome (green) and mitochondria (red) by confocal fluorescence microscopy, 400×, 1000×, n = 3; (F) Representative ROS images, 100×, n = 5. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Journal: Frontiers in Pharmacology

    Article Title: Oxymatrine alleviates cognitive dysfunction in mice caused by chronic cerebral hypoperfusion by promoting SIRT1/ PINK1-mediated mitophagy

    doi: 10.3389/fphar.2026.1736022

    Figure Lengend Snippet: SIRT1 and mitophagy mediate the protective effect of OMT on OGD/R HT22 cells. (A) The mRNA expression of MAP2, PSD95, SYN PINK1 and PARKIN, n = 6; (B,C) The protein expression and quantitative analysis of MAP2, PSD95, SYN PINK1 and PARKIN, n = 3; (D) Detection of JC-1 aggregates (red) and monomers (green) by confocal fluorescence microscopy, 1000×, n = 3; (E) Colocalization of lysosome (green) and mitochondria (red) by confocal fluorescence microscopy, 400×, 1000×, n = 3; (F) Representative ROS images, 100×, n = 5. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Article Snippet: The antibodies of microtubule-associated protein 2 (MAP2, A2572), postsynaptic density protein-95 (PSD95, A6194), synaptophysin (SYN, A6344) and sirtuin 1 (SIRT1, A11267) were sourced from Abclonal Technology Co., Ltd., Wuhan, China.

    Techniques: Expressing, Fluorescence, Microscopy, Control

    SIRT1 mediates the neuroprotective effect and mitophagy of OMT on the hippocampus of CCH mice. (A,B) The representative traces and quantitative analysis in OFT, Y-maze and NOR test, n = 6; (C,D) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6; (E,F) The mRNA expression of MAP2, PSD95, SYN PINK1 and PARKIN, n = 6; (G,H) Representative immunofluorescence images and quantitative analysis of MAP2 and PINK1, n = 5; (I,J) The protein expression and quantitative analysis of MAP2, PSD95, SYN PINK1 and PARKIN, n = 3. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Journal: Frontiers in Pharmacology

    Article Title: Oxymatrine alleviates cognitive dysfunction in mice caused by chronic cerebral hypoperfusion by promoting SIRT1/ PINK1-mediated mitophagy

    doi: 10.3389/fphar.2026.1736022

    Figure Lengend Snippet: SIRT1 mediates the neuroprotective effect and mitophagy of OMT on the hippocampus of CCH mice. (A,B) The representative traces and quantitative analysis in OFT, Y-maze and NOR test, n = 6; (C,D) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6; (E,F) The mRNA expression of MAP2, PSD95, SYN PINK1 and PARKIN, n = 6; (G,H) Representative immunofluorescence images and quantitative analysis of MAP2 and PINK1, n = 5; (I,J) The protein expression and quantitative analysis of MAP2, PSD95, SYN PINK1 and PARKIN, n = 3. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Article Snippet: The antibodies of microtubule-associated protein 2 (MAP2, A2572), postsynaptic density protein-95 (PSD95, A6194), synaptophysin (SYN, A6344) and sirtuin 1 (SIRT1, A11267) were sourced from Abclonal Technology Co., Ltd., Wuhan, China.

    Techniques: Electron Microscopy, Expressing, Immunofluorescence, Control